FRET a la #MullerLab

We had an exciting few weeks demoing a Nikon Ti2E microscope.

The level of cAMP is increasing in these c2c12 cells with pharmacologic modulators of cAMP production/hydrolysis. Here, we are using a cytosolic FRET sensor that will lose the CFP-to-YFP FRET with increasing cAMP level, but the ratio view is pseudo-coloring a view of CFP/YFP ratio so that the intensity increases as this is happening.

Originally tweeted by Grace Muller (@GraceKMuller) on October 17, 2022.

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