We had an exciting few weeks demoing a Nikon Ti2E microscope.
The level of cAMP is increasing in these c2c12 cells with pharmacologic modulators of cAMP production/hydrolysis. Here, we are using a cytosolic FRET sensor that will lose the CFP-to-YFP FRET with increasing cAMP level, but the ratio view is pseudo-coloring a view of CFP/YFP ratio so that the intensity increases as this is happening.
Originally tweeted by Grace Muller (@GraceKMuller) on October 17, 2022.
Muller Lab at LUC 